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Illumina (Solexa) sequencing: Illumina sequencing works by simultaneously identifying DNA bases, as each base emits a unique fluorescent signal, and adding them to a nucleic acid chain.requires less DNA/RNA as input (nanograms of materials are sufficient).it offers single-nucleotide resolution, making it possible to detect related genes (or features), alternatively spliced transcripts, allelic gene variants and single nucleotide polymorphisms.a priori knowledge of the genome or genomic features is not required.In contrast to microarray methods, NGS-based approaches have several advantages including:

NGS can be used to analyse DNA and RNA samples and is a popular tool in functional genomics. Such technologies include: Advantages of NGS These technologies allow for sequencing of DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as such revolutionised the study of genomics and molecular biology. Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies. This project, which used first-generation sequencing, known as Sanger sequencing (the chain-termination method), took 13 years, cost $3 billion and was completed in 2003.Ĭompared to conventional Sanger sequencing using capillary electrophoresis, the short read, massively parallel sequencing technique is a fundamentally different approach that revolutionised sequencing capabilities and launched the second-generation sequencing methods – or next-generation sequencing (NGS) – that provide orders of magnitude more data at much lower recurring cost. The first major foray into DNA sequencing was the Human Genome Project. In contrast to microarray methods, sequence-based approaches directly determine the nucleic acid sequence of a given DNA or cDNA molecule.

Library preparation and sequencing approaches.Improvements on the previous technology.What is Next Generation DNA Sequencing?.Submission of data to a public repository.Biological interpretation of microarray data.Counteracting dye bias effects when using two-colour arrays.